引言提供激发愈伤组织产生、细胞培养和原生质体融合的手段可能扩大当前的树木改良程序。树木育种的主要局限性问题之一是树木生活周期相对缓慢,每世代至少在15至20年之间。在近代育种改良中,种间杂交和肥料应用无疑能提高森林木材和果实的生产力。然而,愈伤组织和细胞培养有利于那些利用常规方法难以生根和嫁接的树木进行快速营养繁殖。选 Introduction Providing means to stimulate callus production, cell culture and protoplast fusion may widen the current tree improvement program. One of the major limitations of tree breeding is the relatively slow life cycle of trees, which is at least 15 to 20 years old for each generation. In modern breeding improvement, interspecific hybridization and fertilizer application can undoubtedly improve the productivity of forest timber and fruit. However, callus and cell culture are favored for rapid vegetative reproduction of trees that are difficult to root and graft using conventional methods. selected