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目的:观察中药复方津力达颗粒联合通心络胶囊对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠胰岛β细胞超微结构损伤情况、胰岛β细胞功能及胰腺组织自噬相关蛋白Beclin-1,Atg7,p62表达的影响。方法:采用高脂喂养结合小剂量链脲佐菌素(streptozotocin,STZ)30 mg·kg-1ip建立T2DM大鼠模型,15只雄性SD大鼠设为正常组正常饲料喂养,75只雄性SD大鼠高脂饲料喂养6周后STZ腹腔注射,根据血糖随机分为:模型组,津力达组(JLD),通心络胶囊组(TXL),联合用药组(JT),吡格列酮组(PLZ),JLD组于模型成功后给予津力达颗粒(3.0 g·kg-1·d-1),TXL组于模型成功后给予通心络胶囊(0.4 g·kg-1·d-1),JT组于模型成功后给予津力达颗粒(3.0 g·kg-1·d-1)加通心络胶囊(0.4 g·kg-1·d-1),PLZ组于模型成功后给予吡格列酮(10 mg·kg-1·d-1),连续ig 6周后,检测空腹血糖及血清胰岛素以计算胰岛β细胞功能指数,电镜观察各组大鼠胰岛β细胞超微结构;Western blot方法检测胰腺组织Beclin-1,Atg7,p62蛋白的表达变化。结果:与正常组相比较,模型组大鼠胰岛β细胞损伤减轻,胰岛β细胞功能指数明显降低,胰腺组织Beclin-1,Atg7蛋白表达明显减少,p62蛋白表达明显增加(P<0.01);与模型组比较,各给药组大鼠胰岛功能指数升高,Beclin-1,Atg7表达升高,p62蛋白表达减少(P<0.01),与JLD,TXL组比较,JT组Beclin-1,Atg7表达升高,p62蛋白表达减少(P<0.05)。结论:津力达颗粒联合通心络胶囊可以提高胰岛β细胞功能指数,其作用可能与促进胰岛自噬有关,而维持胰腺组织自噬水平可能是延缓糖尿病进展的有效措施。
OBJECTIVE: To observe the ultrastructural damage of islet β cells, islet β-cell function and autophagy-related protein Beclin-1 in pancreatic tissues of type 2 diabetes mellitus (T2DM) 1, Atg7, p62 expression. Methods: T2DM rats were induced by high-fat diet combined with low dose streptozotocin (STZ) 30 mg · kg-1 ip. 15 male SD rats were fed with normal diet and 75 male SD rats The rats in high fat diet were injected intraperitoneally with STZ 6 weeks after injection. According to the blood glucose, they were randomly divided into model group, JLD group, TXL group, PLT group, JLD group was given Jinlida granule (3.0 g · kg-1 · d-1) after the model was successful, TXL group was given Tongxinluo capsule (0.4 g · kg-1 · d-1) after successful model, JT The rats in the PLZ group were given pioglitazone 10 (10 g · kg-1 · d-1) and Tongxinluo capsule (0.4 g · kg-1 · d-1) mg · kg-1 · d-1). After 6 weeks, the fasting blood glucose and serum insulin were measured to calculate the function index of islet β cells. The ultrastructure of islet β cells was observed by electron microscope. Beclin-1, Atg7, p62 protein expression changes. Results: Compared with the normal group, the islet β cell lesion was lessened and the islet β cell function index was significantly decreased in the untreated group. The expression of Beclin-1 and Atg7 in pancreatic tissue was significantly decreased and the expression of p62 protein was significantly increased (P <0.01) Compared with JLD and TXL group, the expression of Beclin-1 and Atg7 in JT group was higher than that in JLD group (P <0.01) Increased, p62 protein expression decreased (P <0.05). CONCLUSION: Jinlida granules combined with Tongxinluo capsule can increase the beta cell function index, which may be related to the promotion of islet autophagy. Maintaining pancreatic autophagy may be an effective measure to delay the progression of diabetes.