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目的:通过观察肾康丸对2型糖尿病(DM)大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及肾脏磷酸化信号转导和转录活化因子-3(p-STAT3)表达的影响,探讨其防治糖尿病肾病(DN)的分子生物学机制。方法:采用高脂高糖喂养加腹腔注射30mg/kg链脲佐菌素(STZ)制备2型DM大鼠模型,大鼠随机分为4组:正常对照组(C组)、模型对照组(M组)、依那西普治疗组(DE组)、肾康丸治疗组(DS组)。各组分别干预8周后,检测大鼠尿α1-微球蛋白(Uα1-MG)、血清尿素氮(BUN)、血肌酐(C)r、TNF-α、IL-6水平及p-STAT3蛋白表达。结果:应用肾康丸、依那西普干预后,大鼠一般状况改善,Uα1-MG、BUN、Cr、TNF-α、IL-6水平较模型对照组显著减少(P<0.01),肾组织p-STAT3表达显著降低。结论:肾康丸对2型DM大鼠具有显著的肾保护作用,其作用机制可能与降低大鼠血清TNF-α、IL-6水平,从而影响其下游信号的转导,进而减少肾组织p-STAT3的表达有关。
Objective: To observe the effects of Shenkang pill on serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and renal phosphorylation signal transducers and activators of transcription in Chinese patients with type 2 diabetes mellitus 3 (p-STAT3) expression, to explore its molecular mechanism of prevention and treatment of diabetic nephropathy (DN). Methods: A rat model of Type 2 DM was established by intraperitoneal injection of streptozotocin (STZ) at a dose of 30 mg / kg. The rats were randomly divided into 4 groups: normal control group (C group), model control group M group), Etanercept group (DE group) and Shenkang pill group (DS group). The levels of urinary α1-microglobulin (Uα1-MG), serum urea nitrogen (BUN), serum creatinine (C) r, TNF-α, IL-6 and p-STAT3 expression. Results: After the rats were treated with Shenkang Pills and Etanercept, the general condition of the rats was improved and the levels of Uα1-MG, BUN, Cr, TNF-α and IL-6 were significantly decreased compared with the model control group p-STAT3 expression was significantly reduced. CONCLUSION: Shenkang Pill has a significant renal protective effect on type 2 DM rats, and its mechanism may be related to the decrease of serum TNF-α and IL-6 levels, thereby affecting the downstream signal transduction and further reducing the expression of p -STAT3 expression.