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通过利用 2 2个 10bp随机引物对来自西藏高原地区 10 7份白菜型油菜种质资源材料的PAPD分析 ,探讨了西藏白菜型油菜品种之间的遗传分化关系。结果表明 :(1)供试的 10 7份材料共产生 2 36条谱带 ,其中 2 10条谱带有多态性 ,占 88.98% ,说明白菜型油菜在西藏高原地区具有较丰富的遗传多样性 ;(2 )根据引物扩增出的DNA指纹图谱 ,运用UPGMA分析法 ,在遗传距离为 0 .0 78处 ,可将供试的 10 7份白菜型油菜划分为 11个类群 ,发现来自于同一地区或气候相似区的品种往往聚在一起 ,表明西藏高原白菜型油菜品种的相似性与其原产地的地理、气候背景密切相关。并在此基础上 ,结合西藏高原农业发展历史、气候背景以及地形地貌特点和植物地理学、植物区系学、植物进化论等方面的综合分析 ,提出西藏高原是世界白菜型油菜起源地的观点。
Through the use of 22 bp random primers to analyze the genetic diversity of 107 Chinese cabbage rape germplasm resources from Tibet Plateau, the genetic differentiation between Tibetan cabbage rape cultivars was explored. The results showed that: (1) A total of 236 bands were obtained from 10 of the tested materials, of which 210 bands were polymorphic, accounting for 88.98%, indicating that Brassica campestris has a rich genetic diversity in the Tibetan Plateau (2) According to the DNA fingerprinting amplified by primer, using UPGMA analysis, 107 rapeseed (Brassica napus L.) cultivars could be divided into 11 groups at the genetic distance of 0.78 and found to be from The same region or climate similar areas tend to get together, indicating that the similarities of the Brassica campestris species on the Tibetan plateau are closely related to the geographical and climatic background of their place of origin. On the basis of this, combined with the history of agricultural development in Tibet Plateau, the climatic background, topographic and geomorphological features, plant geography, flora and phylogenetic analysis, the article put forward the view that the Tibetan plateau is the origin of Brassica napus in the world.