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材料是6只孕羊,其中双胎一只、单胎5只,禁食48h脊椎麻醉后静脉注射戊巴比妥钠全身麻醉。切开母羊皮肤插入聚乙烯导管(外直径1.4mm):插入母羊股动静脉、子宫静脉子宫角、胎羊腹主动脉、脐静脉及股静脉、以及羊膜腔内也插入导管供注射抗生素(500mg氨必西林)。手术后止痛剂用2d,孕羊手术后≥4d恢复,孕羊饲标准紫花苜蓿、水及矿物质,进食q12h定量,导管每天用等渗生理盐水肝素冲洗干净,孕羊置室温18℃±2℃。
The material is 6 pregnant sheep, of which twins, 5 singletons, 48h spinal anesthesia after intravenous injection of sodium pentobarbital anesthesia. Polyethylene catheters (1.4 mm outer diameter) were cut into the ewe skin: insertion of ewe femoral vein, uterine horn of uterine vein, fetal abdominal aorta, umbilical vein and femoral vein, and catheterization of the amniotic cavity for injection of antibiotics (500 mg ampicillin). After 2 days of operation, the analgesic agent was recovered for more than 4 days after the operation. The pregnant sheep was fed with standard alfalfa, water and minerals. The rats were fed with q12h, and the catheter was rinsed with isotonic saline heparin. The pregnant sheep were kept at room temperature 18 ℃ ± 2 ℃.