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为了明确M97抗条锈性遗传规律,在苗期用7个小麦条锈菌系对M97与感病品种铭贤169的杂交后代F1、F2、F3和BC1代进行抗条锈性遗传分析,并对M97抗Sun11-4的抗条锈基因进行SSR分子标记。M97对Sun11-4和Sun11-11的抗病性均由1对显性基因控制,对CY29、CY30、CY33的抗病性由1显1隐2对基因共同控制,对CY31的抗病性由2对显性基因独立或重叠作用控制。以接种Sun11-4的F2代分离群体构建作图群体,筛选到Xwmc222、Xwmc147、Xbarc229和Xwmc339等4个与抗病基因连锁的SSR标记,其遗传距离分别为3.4、4.8、7.6和12.1 cM。将该抗病基因定位于小麦1DS染色体,且该基因不同于已知的抗条锈基因,暂命名为YrM97。用YrM97两侧遗传距离最近的2个标记Xwmc222和Xwmc147对42个黄淮麦区主栽小麦品种进行分子检测,仅有9.5%的品种具有与YrM97相同的标记位点。
In order to clarify the genetic rule of stripe rust resistance of M97, seven wheat stripe rust lines were used to analyze the resistance to stripe rust in F1, F2, F3 and BC1 progenies of M97 and susceptible cultivar Mingxian169 The anti-stripe rust gene of M97 anti-Sun11-4 was SSR-labeled. The resistance of M97 to Sun11-4 and Sun11-11 was controlled by one pair of dominant genes. The resistance of CY97 to CY29, CY30 and CY33 was controlled by 1 locus and 2 loci, and the resistance to CY31 was controlled by 2 Control of dominant genes independently or overlappingly. The F2 population of inbred Sun11-4 was used to construct the mapping population. Four SSR markers linked to disease-resistance genes Xwmc222, Xwmc147, Xbarc229 and Xwmc339 were screened with genetic distances of 3.4, 4.8, 7.6 and 12.1 cM, respectively. The resistance gene is located on wheat 1DS chromosome, and the gene is different from the known resistance to stripe rust gene, tentatively named YrM97. 42 cultivars of wheat cultivars from Huang-Huai Wheat Region were detected by using two markers Xwmc222 and Xwmc147 with the closest genetic distance on both sides of YrM97. Only 9.5% of the cultivars had the same marker sites as YrM97.