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目的：研究Ａｄａ蛋白质对ａｌｋＡ基因表达的调节，探讨Ａｄａ蛋白质与ａｌｋＡ基因有效转录的关系。方法：采用ＤＮＡ顺序测定和体外转录的方法，以ｌａｃ基因启动子为对照进行实验。结果：Ａｄａ蛋白质加入以突变和野生ａｌｋＡ基因启动子为模板的体外转录体系中，产生的ａｌｋＡｍＲＮＡ高于无Ａｄａ蛋白质组；Ａｄａ蛋白质加入ｌａｃ基因启动子转录体系中，产生的ｌａｃｍＲＮＡ与未加Ａｄａ蛋白质组基本相同。结论：Ａｄａ蛋白质对ｌａｃ基因启动子转录调节作用不明显，对于突变的和野生的ａｌｋＡ基因转录均有促进作用，这种调节作用可能发生在启动子上游区。
AIM: To investigate the regulation of alka gene expression by Ada protein and to explore the relationship between Ada protein and alkA gene transcription. Methods: DNA sequence determination and in vitro transcription method, lac gene promoter as a control experiment. Results: Ada protein was added into the in vitro transcription system with mutant and wild-type alkA promoter as template. The resulting alkA mRNA was higher than that without Ada protein. When Ada protein was added into the lac gene promoter transcription system, laccRNA produced was significantly higher than that without Ada protein The group is basically the same. CONCLUSION: The Ada protein has no obvious effect on the transcriptional regulation of lac gene promoter, which can promote the transcription of mutant and wild alkA gene. This regulation may take place in the promoter upstream region.