初步研究了盐分胁迫对水稻幼苗和愈伤组织生长的影响,并探讨了外源脯氨酸的作用。结果表明,水稻幼苗和愈伤组织的生长,随氯化钠浓度升高均而明显降低。在盐分胁迫下幼苗中积累游离脯氨酸,并随盐浓度升高而增加。籼稻品种脯氨酸积累较粳稻品种多。脯氨酸积累量与幼苗受盐害程度呈相反趋势。过氧化物酶活性在盐浓度为1.5%时最强。该酶活性变化与幼苗受害程度未见明显联系。过氧化物酶和细胞色素氧化酶分布在幼苗主根横切面上。不同处理间无明显差异。外源脯氨酸能明显减轻盐分对愈伤组织生长的抑制,但对幼苗生长影响不明显。在无盐分胁迫下脯氨酸明显促进水稻愈伤组织的生长。 The effects of salt stress on the growth of rice seedlings and callus were studied. The effects of exogenous proline were also studied. The results showed that the growth of rice seedlings and callus decreased significantly with the increase of sodium chloride concentration. Accumulation of free proline in seedlings under salt stress increased with increasing salt concentration. Indica rice had more proline accumulation than japonica rice. The accumulation of proline showed the opposite trend with the degree of salt damage by seedlings. Peroxidase activity was the strongest at a salt concentration of 1.5%. No significant correlation was observed between the change of the enzyme activity and the degree of seedling damage. Peroxidase and cytochrome oxidase distribution in the seedling root cross-section. No significant difference between different treatments. Exogenous proline can significantly reduce salt inhibition of callus growth, but the impact on seedling growth is not obvious. Proline significantly promoted the callus growth of rice under salt-free stress.