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目的:了解建德市宿主动物钩端螺旋体菌群分布和变动情况,为研究钩体病流行规律,制定预防和控制措施提供依据。方法:利用Korthof培养基和暗视野显微镜(DGM)观察有无钩端螺旋体生长,并做血清学分型鉴定。结果:从77只青蛙标本中分离到钩体菌3株,阳性率为3.90%,均为黄疸出血群。结论:青蛙是我市钩端螺旋体病的重要宿主,黄疸出血群是宿主动物感染的主要菌群,与1989年监测资料,未发生菌群更迭现象。虽然连续12年无病例发生,仍需积极防范。
OBJECTIVE: To understand the distribution and changes of Leptospira bacteria in Jiande City, and to provide basis for the study of the prevalence of Leptospirosis and formulation of prevention and control measures. Methods: The growth of Leptospira was observed with Korthof medium and dark field microscopy (DGM), and serological typing was performed. Results: Three strains of Leptospira were isolated from 77 frog specimens, the positive rate was 3.90%. All of them were jaundice bleeding. Conclusion: The frog is an important host of Leptospirosis in our city. The jaundice hemorrhage is the main flora infected by the host animal. With the monitoring data in 1989, no phenomenon of flora change occurred. Although 12 consecutive cases of non-occurrence, still need to actively prevent.