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[目的]探讨甘麦大枣汤对慢性应激抑郁模型大鼠行为活动、学习记忆及海马突触可塑性的影响.[方法]将Wistar大鼠随机分为4组,即空白组、抑郁模型组、氟西汀组(5.4 mg/kg)、甘麦大枣汤组(3.84 g/kg),采用慢性温和不可预见性应激加孤养的方式建立抑郁模型,造模同时灌胃(ig)给药,连续21 d,观察甘麦大枣汤对模型大鼠水迷宫测试的影响,采用免疫组化、Western-Blotting的方法检测各组大鼠海马突触素I(SYNⅠ)、突触素(SYNA)、脑源性神经营养因子(BDNF)的表达水平并比较.[结果]与正常组比较,模型组大鼠水平及垂直活动的得分明显下降(P<0.01),逃避潜伏期(EL)、目标象限潜伏时间显著增长(P<0.05),SYNⅠ、SYNA、BDNF的表达显著下降;与模型组的结果比较,甘麦大枣汤能缩短模型大鼠的EL(P<0.05),并能缩短目标象限潜伏时间(P<0.05),增加模型大鼠海马SYNⅠ和SYNA的表达(P<0.01或P<0.05),促进BDNF的含量增加.[结论]甘麦大枣汤能显著改善抑郁模型大鼠的学习记忆能力,其机制可能是通过提升其海马突触可塑性而发挥作用.“,”[Objective]To investigate the effects of Ganmai Dazao Decoction on learning,memory and hippocampus synaptic plasticity in chronic depression model rats.[Methods]Wistar rats were randomly divided into 4 groups: the control group,he depression model group,the fluoxetine group (5.4mg/kg) and the Ganmai Dazao Decoction (3.84 g/kg) group.Depression models were established by chronic mild unpredictable stress and solitude.The fluoxetine hydrochloride and Ganmai Dazao Decoction group were intra-gastrically administrated (ig) with corresponding drugs once a day for 21 days,and the depression model group was intraperitoneally injected with water.Then,we observed the effects of water maze test in model rats with Ganmai Dazao Decoction.The expression of SYN I (synaptophysin I),SYNA (synaptophysin),and BDNF (brain-derived neurotrophic factor) were detected by the immunohistochemistry and Western-Blotting methods.[Results]Compared to the control group,the horizontal and vertical activity scores of the model group decreased significantly (P<0.01).However,the escape latency (EL) and target quadrant latency increased significantly (P<0.05),and the expression of SYN I,SYNA,BDNF were reduced dramatically as well.Compared to the outcomes of the model group,Ganmai Dazao Decoction reduced the EL (P<0.05),shortened the target quadrant latency (P<0.05) and increased the expression of hippocampal SYN I,SYNA and BDNF(P<0.01 or P<0.05) of the model rats.[Conclusion]Ganmai Dazao Decoction can significantly improve the learning and memory abilities in chronic depression model rats,which may play a role in the promotion of hippocampal synaptic plasticity.