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目的建立RP-HPLC双波长同时测定熟地黄中4种成分含量的方法。方法色谱柱为WatersSunfire C18柱(4.6 mm×250 mm,5μm);以乙腈-体积分数0.2%磷酸水溶液为流动相,梯度洗脱;检测波长为λ1=210 nm和λ2=330 nm;流速为1.0 mL.min-1;柱温为30℃。结果梓醇、麦角甾苷、吉奥诺苷B1和马替诺皂苷质量浓度分别在125.0～2 000.0 mg.L-1(r=0.999 9)、11.0～176.0mg.L-1(r=0.999 9)、15.0～240.0 mg.L-1(r=0.999 9)和7.5～120.0 mg.L-1(r=0.999 8)内与峰面积呈良好的线性关系。加样回收率在97.5%～101.3%内。结论该方法灵敏,操作简便,结果可靠,可用于熟地黄药材的质量控制。
OBJECTIVE To establish a dual-wavelength RP-HPLC method for simultaneous determination of four components in Radix Rehmanniae Preparata. Methods The column was Waters Sunfire C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution. The detection wavelength was λ1 = 210 nm and λ2 = 330 nm. mL.min-1; the column temperature is 30 ℃. Results The concentrations of catalpol, ergosteroid, jinonoside B1 and mannitol were 125.0-2 000.0 mg.L-1 (r = 0.999 9) and 11.0-176.0 mg.L-1 (r = 0.999 9), 15.0 ~ 240.0 mg.L-1 (r = 0.999 9) and 7.5 ~ 120.0 mg.L-1 (r = 0.999 8). The recovery rate was within 97.5% ~ 101.3%. Conclusion The method is sensitive, easy to operate and reliable. It can be used for the quality control of Rehmannia glutinosa.