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The expressions are the mirrors of the cultural diversities, and usually different cultural connotations bring them quite surprising associations. So it is important to analyze the similarities and differences of cultural connotations in different languages. With animal expressions as target, the paper analyzes cultural connotations in the expressions involving animals in English and Chinese comparatively by a number of examples. This may help people gain a comprehensive and deeper insight into different cultural connotations of English and Chinese idioms, and further improve the communication of the two cultures.