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研究兔髂动脉内皮剥脱时血管壁细胞的反应特点和 c- myc基因的表达规律。用标准血管成形导管髂动脉球囊损伤 1 2只家兔的右髂总动脉后 ,分别于 3、7、40 d处死动物 ,用透射电镜观察术后 3d平滑肌细胞 ( SMC)超微结构的变化 ,光镜观察术后血管壁细胞的反应特点和 c- myc蛋白表达规律 ,同时对术后 40 d动物行血管造影。结果发现 :术后 3d兔髂总动脉中层 SMC由收缩型转变为分泌型 ,并向内膜下迁移 ,可见管腔内有血栓形成 ,1周时弹力板不完整 ,中膜 SMC排列紊乱 ,术后 40 d增生的 SMC和细胞外基质形成新生内膜 ,血管造影显示管腔狭窄 5 0 %～ 80 % ;球囊损伤段髂总动脉新生内膜 c- myc蛋白免疫组化染色为阳性 ,而对照组为阴性。结论 :球囊损伤髂动脉后可引起 SMC迁移、增生 ,其和细胞外基质一起形成新生内膜 ,管腔狭窄 ;SMC增生和 c- myc基因表达密切相关。
To study the response characteristics of vascular wall cells and the expression of c-myc gene in the rabbit iliac artery endothelial stripping. The animals were sacrificed at 3, 7, and 40 days after the common iliac artery balloon injury to the right common iliac artery in rabbits with standard angioplasty catheter. The ultrastructural changes of smooth muscle cells (SMCs) were observed by transmission electron microscopy Light microscopy was used to observe the response characteristics of the vascular wall cells and the expression of c-myc protein. Angiography was performed on the animals 40 days after operation. The results showed that the SMC of middle iliac artery changed from contractile to secretive and migrated subintimally, showing thrombus formation in the lumen at 3d postoperatively. One week later, the elastic plate was incomplete and the tunica media was disordered. After 40 days, SMMCs and extracellular matrix formed neointimal hyperplasia, angiography showed lumen stenosis 50% ~ 80%; balloon injury section of common iliac artery neointimal c-myc protein immunohistochemical staining was positive, and The control group was negative. CONCLUSIONS: The injured iliac arteries of the balloon can cause the migration and proliferation of SMC, which together with the extracellular matrix form neointimal and stenosis; SMC hyperplasia and c-myc gene expression are closely related.