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通过动物接种方法研究血管内皮细胞生长因子 (VEGF)抗体对骨肉瘤生长的抑制作用。将OS 732骨肉瘤细胞以 1 0 70 / (mL·只 )的剂量接种于BALB/C型裸鼠腋窝部皮下 ,并分成 4组 :A组于接种次日于瘤体局部注射VEGF抗体 ,B组于接种后 2周瘤体局部注射抗体 ,C组于接种次日于瘤体局部注射PBS ,D组不注射抗体。VEGF注射剂量为 2 0 0 μg/ (只·次 ) ,每周 3次 ,共 3周。 4周后处死A、C2组裸鼠 ,6周后处死B、D 2组裸鼠。每组均取出瘤组织作病理切片。计算肿瘤体积及肿瘤内微血管密度 ,并对结果进行分析。结果显示 ,A组的肿瘤体积小于B、C、D 3组 ,在接种后 2 5d与D组有统计学差异 (P <0 .0 5 ) ;B、C、D 3组间无统计学差异。 4组肿瘤内微血管密度的比较 ,无统计学差异。提示 ,VEGF抗体对骨肉瘤早期的生长有明显的抑制作用 ,可作为转移病灶的预防及治疗方法之一。
To study the inhibitory effect of VEGF antibody on the growth of osteosarcoma by animal inoculation method. OS 732 osteosarcoma cells were inoculated subcutaneously into the axilla of BALB / c nude mice at a dose of 1070 cells / mL and divided into 4 groups: Group A received local injection of VEGF antibody on the next day Group 2 weeks after inoculation tumor injection of local antibodies, C group on the day after inoculation in the tumor local injection of PBS, D group does not inject antibodies. VEGF injection dose of 200 μg / (only · times), 3 times a week for 3 weeks. After 4 weeks, A and C2 nude mice were killed, and after 6 weeks, nude mice in groups B and D 2 were sacrificed. Tumors were removed from each group for biopsy. Tumor volume and intracranial microvessel density were calculated and the results analyzed. The results showed that the tumor volume in group A was smaller than that in group B, C and D 3, and there was a significant difference between group D and group D at 25 days after inoculation (P <0.05). There was no significant difference between group B, C and D 3 . 4 groups of tumor microvessel density comparison, no statistical difference. Tip, VEGF antibody on the early growth of osteosarcoma was significantly inhibited, as one of the prevention and treatment of metastatic lesions.