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目的:探讨雌激素对基质细胞衍化因子-1(SDF-1)的影响。方法:选取乳腺癌细胞系MCF-7和MDA-MB-231为研究对象,分成对照组、雌激素组和雌激素+雌激素受体阻断组,分别加入不同生理浓度的17-β雌二醇作用一定时间以及同一浓度17-β雌二醇作用不同时间点,用酶联免疫吸附实验(ELISA)方法测定培养液中SDF-1的浓度,半定量逆转录聚合酶链反应(RT-PCR)法检测细胞SDF-1 mRNA的表达。结果:MDA-MB-231细胞系加与未加雌激素,均未检测到SDF-1的分泌。而MCF-7细胞基础培养液中可检测到SDF-1分泌。不同生理浓度的17-β雌二醇均可增加MCF-7细胞SDF-1的分泌水平。当加入1×10-7mol/L的生理高浓度17-β雌二醇时,细胞分泌SDF-1水平在2 h达到高峰,是对照组的6倍〔(1 823.17±325.18)ρg/mLvs(307.23±5.42)ρg/mL,F=201.02,P<0.01〕,该作用可被雌激素受体拮抗剂(ICI182,780)消除。此外,SDF-1mRNA的表达水平与测得的SDF-1蛋白水平相一致。结论:在某些乳腺癌细胞系,生理浓度的雌激素可促进SDF-1的分泌,而这种作用主要是通过雌激素受体来实现。雌激素可通过SDF-1来影响乳腺癌的生物学特性。
Objective: To investigate the effect of estrogen on stromal cell-derived factor-1 (SDF-1). Methods: The breast cancer cell lines MCF-7 and MDA-MB-231 were selected as the research object and divided into control group, estrogen group and estrogen + estrogen receptor block group, respectively, with different physiological concentrations of 17- Alcohol concentration for a certain time and the same concentration of 17-β-estradiol at different time points, the concentration of SDF-1 in the culture medium was determined by enzyme-linked immunosorbent assay (ELISA) method. Semiquantitative reverse transcription polymerase chain reaction ) Method was used to detect the expression of SDF-1 mRNA. Results: No secretion of SDF-1 was detected in MDA-MB-231 cell line with and without estrogen. However, SDF-1 secretion was detected in the basal medium of MCF-7 cells. 17-β estradiol at different physiological concentrations increased the secretion of SDF-1 in MCF-7 cells. SDF-1 secreted by the cells peaked at 2 h after adding 1 × 10-7mol / L 17-β-estradiol, which was 6 times higher than that of the control [(1823.17 ± 325.18) ρg / mL vs ( 307.23 ± 5.42) ρg / mL, F = 201.02, P <0.01〕. This effect can be eliminated by the estrogen receptor antagonist (ICI182,780). In addition, the expression level of SDF-1 mRNA is in agreement with the measured SDF-1 protein level. Conclusion: In some breast cancer cell lines, physiological concentrations of estrogen can promote SDF-1 secretion, and this effect is mainly achieved through the estrogen receptor. Estrogen can affect the biological characteristics of breast cancer through SDF-1.