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目的:孕妇饮酒是造成胎儿酒精中毒症的直接原因,会导致头过小(mi-crocephaly)及智力障碍.人脑的发育有两个高峰期,其中妊娠后3月至生后18月为脑生长高峰期(brain growth spurt),此期人脑对酒精毒性最为敏感.本研究意在探索胎儿脑酒精中毒的细胞分子机制.方法 大鼠脑生长高峰是在生后5～16天,我们选择生后 6～8天的SD大鼠20只,分为急性酒精中毒组(腹腔注射2000 proof ethanol 2.5g/kg)及对照组(腹腔内注射同等剂量的生理盐水).酒精作用后6小时快速断头取脑,分离两侧海马组织共100mg,经SDS,Proteinase K等消化,用SIGMA TACS~(TM)Apoptotic DNA laddering kits提取海马细胞DNA,1.5%Agarose Gel水平电泳仪电泳分离凋凋亡细胞核小体间的DNA片段,EB染色,UV观察.结果:酒精中毒组海马细胞的DNA片段呈现以200bp为倍数的规律,在凝胶上显出明显的梯度.而对照组的
Aims: Pregnant women drink alcohol is the direct cause of fetal alcoholism, can lead to mi-crocephaly and mental retardation.Human brain development has two peak periods, of which from March to 18 after pregnancy Brain growth spurt, the human brain is most sensitive to alcohol toxicity.The purpose of this study is to explore the cellular molecular mechanism of fetal brain alcoholism.Methods Rat brain growth peak is 5 to 16 days after birth, we choose 20 SD rats aged 6 to 8 days after birth were divided into acute alcoholism group (2000 proof ethanol 2.5g / kg) and control group (intraperitoneal injection of the same dose of saline) .After 6 hours, A total of 100mg of hippocampus was isolated from both sides of the hippocampus. The cells were digested with SDS, Proteinase K, and the DNA of hippocampus was extracted with SIGMA TACS ™ Apoptotic DNA laddering kits. Apoptotic apoptotic nuclei were separated by 1.5% Agarose Gel electrophoresis. EB DNA, EB staining and UV observation.Results: The DNA fragment of hippocampus in alcoholism showed a rule of multiples of 200bp, showing a clear gradient on the gel, while in the control group