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目的：建立人卵巢癌体外耐药模型，研究卵巢癌对阿霉素的耐药特征和机制。万法；应用浓度递增和短时间作用法，从人卵巢癌细胞A2780中培养出对阿霉素耐药细胞亚株（A2780／ADM）。采用MTT法检测耐药细胞的耐药指数及对抗癌药物的敏感性，用高效液相色谱仪捡测耐药细胞内阿霉素含量，以及应用RT-PCR法和免疫组化法检测MDR1和GST－π的表达情况。结果：（1）A2780／ADM对ADM的耐药指数为38．2，而且其倍增时间较A2780细胞延长，对VCR、MTX呈高度耐药状态，对KSM、CARP、PYM、VP16、CTX和5－FU均有不同程度的耐药。（2）耐药细胞内阿霉素含量明显低于A2780细胞。（3）耐药细胞MDR1的表达呈阳性，而GST－π只有在高浓度的耐药细胞内才呈弱阳性。结论：A2780细胞对阿霉素的耐药是获得性的，并呈多药耐药特征，其耐药的主要原因是细胞内MDR1的过度表达，导致药物在耐药细胞内浓度降低。此项研究结果有助于卵巢癌患者化疗选用药物时作为参考。
OBJECTIVE: To establish an in vitro drug resistance model of human ovarian cancer and investigate the characteristics and mechanism of drug resistance to doxorubicin in ovarian cancer. Method: The adriamycin-resistant cell subline (A2780 / ADM) was cultured from human ovarian cancer cell A2780 with increasing concentration and short-term effect. The drug resistance index of drug-resistant cells and the sensitivity to anticancer drugs were detected by MTT assay. The content of doxorubicin in drug-resistant cells was detected by high performance liquid chromatography (HPLC) and the expression of MDR1 was detected by RT-PCR and immunohistochemistry And GST-π expression. Results: (1) The drug resistance index of A2780 / ADM to ADM was 38.2, and its doubling time was longer than that of A2780 cells, and was highly resistant to VCR and MTX. The resistance index to KSM, CARP, PYM, VP16, CTX and 5 -FU has varying degrees of resistance. (2) The drug resistant intracellular doxorubicin content was significantly lower than A2780 cells. (3) The expression of MDR1 in drug-resistant cells was positive, while GST-πwas weakly positive only in high concentration of drug-resistant cells. CONCLUSION: A2780 cells are resistant to doxorubicin and have multidrug resistance characteristics. The main reason of resistance to doxorubicin is over-expression of MDR1 in cells, leading to the decrease of concentration of drug in resistant cells. The results of this study help ovarian cancer chemotherapy selection of drugs as a reference.