探索了在麦角菌中进行省却营养缺陷型标记的种间灭活原生质体融合。亲株Ⅰ（Ｃ．ｐｕｒｐｕｒｅａ，麦角隐亭产生菌）用紫外线灭活（１５Ｗ，照射距离３０ ｃｍ ）７５ ｍ ｉｎ，存活率约６×１０－ ５；亲株Ⅱ（Ｃ．ｐａｓｐａｌｉ，麦角酰胺产生菌）用热灭活（５０～５２℃）６０ ｍ ｉｎ，存活率为零。融合后的再生菌落均为亲株Ⅰ的形态，其再生频率比灭活的亲株Ⅰ提高约２０ 倍，推测绝大多数为融合子。后者经５ 次传代，大多数的产碱能力与亲株Ⅰ相当或略有提高。 Exploring Interspecies Inactivation of Protoplast Fusion with Ergot-auxin-deficient Marker in Ergot. The parental strain I (C.purpurea) was inactivated by UV light (15 W for a distance of 30 cm) for 75 mins with a survival rate of about 6 × 10-5. The bacteria were heat-inactivated (50-52 ° C) for 60 min and the viability was zero. The regenerated colonies after the fusion all had the form of the parent strain Ⅰ, and the frequency of regeneration was about 20 times higher than that of the inactivated parent strain I, suggesting that most of them were fusions. The latter pass 5 times, most of the alkali-producing capacity and parental Ⅰ comparable or slightly increased.