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本文作者用脐带血T淋巴细胞和成人末梢血T淋巴细胞分别与绵羊红细胞做E花环形成试验.试验结果发现脐带血E花环阴性的T淋巴细胞(TE-)高于末梢血E花环阴性的T淋巴细胞,前者阳性率为30%,后者为13%.脐带血TE-细胞99%OKT_3阳性.用溴化二氨基乙基异硫脲(AET)处理绵羊红细胞,再与脐带血TE-细胞做AET花环试验,95%TE-细胞可形成AET花环(AET~+).由此可见脐带血T细胞有TE-/AET~+的特性.一般常依据5-核苷酸酶水平高低作为细胞成熟与否的标志,因此作者检测了脐带血中TE-/AET~+细胞内的5-核苷酸酶水平.
In this study, cord blood T lymphocytes and adult peripheral blood T lymphocytes were used to make erythrocytes with sheep erythrocytes E-ring formation test results showed that cord blood E-rosette-negative T lymphocytes (TE-) higher than the peripheral blood E-ring-negative T Lymphocytes, the former positive rate was 30%, the latter was 13% .The umbilical cord blood TE-cells 99% OKT_3positive.Methods The sheep erythrocytes were treated with aminoethanethiourea bromide (AET) AET garland test, 95% TE- cells can form AET garland (AET ~ +) .Therefore, umbilical cord blood T cells have TE- / AET ~ + characteristics.Usually often based on the level of 5-nucleotidase as a cell Therefore, the authors examined the level of 5-nucleotidase in TE- / AET ~ + cells in umbilical cord blood.