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目的 探讨血管紧张素转换酶抑制剂（ Ａ Ｃ Ｅ Ｉ） 苯那普利对单侧肾切除糖尿病大鼠肾皮质血小板衍化生长因子 Ｂ（ Ｐ Ｄ Ｇ Ｆ Ｂ） 及其β受体表达的影响。方法 苯那普利（１０ｍｇ·ｋｇ１·ｄ１ 灌胃） 治疗４ 周后应用荧光分光光度法检测各组血浆和肾皮、髓质 Ａ Ｃ Ｅ 活性；用免疫组织化学方法检测肾皮质Ⅳ型胶原、纤维连接蛋白（ Ｆ Ｎ） 及 Ｐ Ｄ Ｇ Ｆ Ｂ 蛋白表达；用 Ｎｏｒｔｈｅｒｎ 和 Ｗｅｓｔｅｒｎ 杂交检测肾皮质 Ｐ Ｄ Ｇ Ｆ Ｂｍ Ｒ Ｎ Ａ 及细胞膜 Ｐ Ｄ Ｇ Ｆβ受体蛋白表达。结果 苯那普利能降低糖尿病大鼠血肌酐、相对肾重及肾皮质 Ａ Ｃ Ｅ 活性。免疫组织化学结果提示苯那普利可抑制糖尿病肾皮质肾小球及肾小管Ⅳ型胶原、 Ｆ Ｎ 及 Ｐ Ｄ Ｇ Ｆ Ｂ 蛋白高表达； Ｎｏｒｔｈｅｒｎ 杂交表明肾皮质 Ｐ Ｄ Ｇ Ｆ Ｂ ｍ Ｒ Ｎ Ａ 表达比对照组增加近３ 倍， Ｗｅｓｔｅｒｎ 杂交证实肾皮质细胞膜 Ｐ Ｄ Ｇ Ｆβ受体蛋白表达比对照组增加近１ ．５ 倍。苯那普利对肾皮质 Ｐ Ｄ Ｇ Ｆ Ｂｍ Ｒ Ｎ Ａ 表达无明显影响，但它可明显抑制肾皮质细胞膜 Ｐ Ｄ Ｇ Ｆβ受体蛋白高表达。结论 苯那普利对糖尿病大鼠肾脏保护作用至少部分与抑制 Ｐ Ｄ Ｇ Ｆ?
OBJECTIVE: To investigate the effect of angiotensin converting enzyme inhibitor (A C E I) benazepril on the expression of platelet-derived growth factor-B (β-DGFP) and β receptor in renal cortex of unilateral nephrectomized diabetic rats influences. Methods Benazepril (10mg · kg1 · d1 intragastric administration) for 4 weeks after treatment by fluorescence spectrophotometry to detect the plasma and renal cortex, medulla A C E activity; immunohistochemical detection of renal cortex Type Ⅳ collagen, fibronectin (F N) and P D G F B protein expression; using Northern and Western hybridization detection of renal cortex P D G F Bm R N A and cell membrane P D G F β receptor protein expression . Results Benazepril reduced serum creatinine, relative renal weight and renal C A activity in diabetic rats. The results of immunohistochemistry suggested that benazepril could inhibit the expression of type IV collagen, FN and PDGF-B protein in diabetic nephropathy glomeruli and tubules. Northern blot showed that the renal cortex P D G F B R N A expression than the control group increased nearly 3-fold, Western blot confirmed renal cortex cell membrane P D G F β receptor protein expression in the control group increased nearly 1. 5 times. Benazepril had no significant effect on the expression of P D G F Bm R N A in renal cortex, but it could significantly inhibit the high expression of P D G F β receptor protein in renal cortex cell membrane. Conclusion Benazepril protects the kidney of diabetic rats at least partly and inhibits P D G F?