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采用无血清组织培养和台盼蓝排斥实验,从细胞形态、细胞成活率等方面分别研究了氯化三丁基锡(TributyltinChloride,TBTCl)对牡蛎鳃组织细胞的影响。实验结果表明,TBTCl对牡蛎鳃细胞的生长及活力均产生一定的影响,具有抑制牡蛎鳃细胞增生,造成牡蛎鳃细胞损伤以及致使鳃细胞死亡的作用。
The effects of Tributyltin Chloride (TBTCl) on the gill cells of oyster were studied respectively from cell morphology and cell viability using serum-free tissue culture and trypan blue exclusion. The results showed that TBTCl had some effects on the growth and vitality of oyster gill cells, and could inhibit the proliferation of oyster gill cells and cause gill cell damage and gill cell death in oysters.