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设计以不同光照强度和pH值等生态因子栽培绞股蓝，当相对照度为39．85％～80．39％时，为绞股蓝生长发育适宜光照，尤以相对照度为64．93％～74．51％时，干物质积累最多，总皂甙含量最高，果实和种子量最大；当相对照度＞80％或＜40％时，干物质积累量大幅度下降，总皂甙含量较低，且当年不能开花结果。在pH值为5．5～8．0的完全培养液（MS）中，绞股蓝茎段均能成活，而以pH值为6．5～7．0时生长发育最佳，并于秋天开花结果，总皂甙含量较高；而在pH值＜5．5或＞8．0时，其插条先后枯黄而死。
The design of Gynostemma pentaphyllum with different light intensity and pH values and other ecological factors, when the relative degree of 39.85% to 80.39%, suitable for the growth and development of Gynostemma pentaphylla, especially in the relative degree of 64.93% ~ 74.51% At the time, dry matter accumulation was the highest, the total saponin content was the highest, and the amount of fruits and seeds was the highest. When the relative level was >80% or <40%, the dry matter accumulation decreased significantly, the total saponin content was low, and the fruit could not be bloomed in the year. In the complete medium (MS) with a pH value of 5.5-8.0, the Gynostemma pentaphyllum leaves survived, but the best growth and development occurred at pH 6.5-7.0, and flowering in autumn. The total saponin content is high; and when the pH value is <5.5 or> 8.0, the cuttings are successively yellow and die.