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描述了一种将乙酰胆碱酯酶固定在SnSe2空心球上检测辛硫磷的简单方法,用水热法合成了SnSe2空心球,并用透射电镜对其表征.固定的乙酰胆碱酯酶能保持其生物学活性,催化乙酰胆碱为胆碱,胆碱被氧化产生可检测的信号.基于辛硫磷对乙酰胆碱酯酶活性有抑制作用这个机理,在理想条件下,这种传感器对辛硫磷检测的线性范围是0.008～56μg/mL,检测限为0.004μg/mL.这种新型的传感器有很好的稳定性和重现性.这项工作表明SnSe2空心球可以作为固定乙酰胆碱酯酶的理想载体并用于构建相应的传感器.
A simple method of immobilizing acetylcholinesterase on the spheres of SnSe2 to detect phoxim was described. SnSe2 hollow spheres were synthesized by hydrothermal method and characterized by transmission electron microscopy. The immobilized acetylcholinesterase retained its biological activity, Catalyzed the acetylcholine choline, choline is oxidized to produce a detectable signal.Based on phoxim on acetylcholinesterase activity inhibition of this mechanism, under ideal conditions, the linear range of this sensor for phoxim detection is 0.008 ~ 56μg / mL, the detection limit is 0.004μg / mL.The new sensor has good stability and reproducibility.This work shows that SnSe2 hollow spheres can be used as an ideal carrier of acetylcholinesterase and used to construct the corresponding sensor .