根据资料记载凋亡细胞的染色可以用吉姆萨染色法[1],但具体的操作方法却未见报道。我们根据吉姆萨染料染血涂片的原理,将其用于大鼠大脑海马区细胞的染色,探索出其染色程序,发现染色效果优于常规HE染色,核仁和凋亡细胞着色清晰,细胞与背景对比明显。现介绍如下:1试剂配制[2]分 Apoptotic cells can be stained by Giemsa staining according to the data [1], but the specific operation has not been reported. According to the principle of Giemsa staining, we used it in the staining of hippocampus cells of rat brain and explored its staining procedure. It was found that the staining effect was better than that of conventional HE staining, the nucleoli and apoptotic cells were well colored, The background contrast. Are introduced as follows: 1 reagent preparation [2] points