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在胚胎发生过程中,肝脏和腹侧胰腺来源于胚胎内胚层的同一细胞群,可以假定胰腺和肝脏内的上皮细胞群可能来源于共同的干细胞群,已有证据表明胰腺干细胞拥有分化成肝细胞的能力,然而,肝脏干细胞分化成胰腺细胞(特别是内分泌胰腺细胞)的能力还不为人所知。 材料和方法:按Petersen等所描述的步骤用2-乙酰胺基笏/肝损伤模型在体内激活肝卵圆细胞,然后用Seglen的二步胶原酶灌注法分离,再通过细胞分类纯化获得Thyl.1-阳性细胞群,纯化的Thyl.1-阳性细胞培养在改进的DMEM
During embryogenesis, the liver and ventral pancreas are derived from the same cell population of embryonic endoderm. It is hypothesized that the epithelial cell population in pancreas and liver may originate from a common stem cell population. There is evidence that pancreatic stem cells have the ability to differentiate into hepatocytes However, the ability of liver stem cells to differentiate into pancreatic cells, especially endocrine pancreatic cells, is unknown. Materials and Methods: Hepithelial oocytes were activated in vivo using a 2-acetamide group / liver injury model as described by Petersen et al., Then isolated by Seglen’s two-step collagenase perfusion method and purified by cell sorting to obtain Thyl. 1-positive cell population, Purified Thyl.1-positive cells were cultured in modified DMEM