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目的:研究汉黄芩素脂质体在小鼠体内的组织分布情况。方法:采用高效液相色谱法[Lichrospher ODS(150 mm×4.6mm,5!m)色谱柱,甲醇-水-乙酸(63∶37∶0.5)为流动相,流速1 mL·min-1,检测波长275 nm,柱温35℃]测定汉黄芩素在小鼠血浆及各组织(心、肝、脾、肺、肾)中的浓度,分别考察汉黄芩素溶液剂和脂质体在小鼠体内的组织分布,运用相对摄取率Re、靶向效率Te和相对靶向效率RTe 3个指标评价汉黄芩素脂质体的靶向性。结果:脂质体组汉黄芩素在肝部的各时间点浓度都明显高于溶液组;脂质体组在肝和脾组织的AUC和MRT增加最为明显,3个靶向评价指标都显示出汉黄芩素脂质体对肝的靶向性显著增强(P<0.01)。结论:对脂质体的靶向性进行评价,结果表明将汉黄芩素包裹于脂质体后,汉黄芩素在肝部位的靶向性显著增强,有利于其疗效的发挥。
Objective: To study the tissue distribution of wogonin liposome in mice. Methods: The mobile phase consisted of Lichrospher ODS (150 mm × 4.6 mm, 5 μm), methanol-water-acetic acid (63:37:0.5) and flow rate of 1 mL · min-1 Wavelength of 275 nm, column temperature of 35 ℃] were used to determine the concentration of wogonin in the plasma and tissues (heart, liver, spleen, lung and kidney) of mice. The concentrations of wogonin solution and liposome were determined in mice The tissue distribution of Wogonin liposomes was evaluated by using three indicators of relative uptake Re, target efficiency Te and relative target efficiency RTe. Results: The concentration of wogonin in the liposome group was significantly higher than that of the solution group at each time point in the liver. The AUC and MRT of the liposome group increased most obviously in the liver and spleen tissues, and the three target evaluation indexes all showed Wogonin liposomes targeting the liver was significantly enhanced (P <0.01). Conclusion: The targeting of liposomes was evaluated. The results showed that the targeting of wogonin in the liver site was significantly enhanced after the wogonin was encapsulated in liposomes, which is beneficial to the therapeutic effect.