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目的:观察紫花牡荆素(Casticin,CAS)诱导人宫颈癌HeLa细胞凋亡作用。方法:体外培养HeLa细胞。ELISA法测定细胞培养液组蛋白/DNA碎片。碘化丙啶(PI)染色流式细胞术(FCM)分析Sub-G1细胞百分率。ELISA法检测细胞Caspase-3活性。结果:CAS以浓度依赖方式增加HeLa细胞组蛋白/DNA碎片的渗漏(P<0.05),同时显著升高HeLa细胞Sub-G1百分率(P<0.05)。CAS以Caspase依赖方式激活HeLa细胞Caspase-3(P<0.05)。结论:CAS能有效诱导HeLa细胞凋亡。
Objective: To observe the apoptosis of HeLa cells induced by Casticin (CAS). Methods: HeLa cells were cultured in vitro. Histone / DNA fragmentation in cell culture medium was measured by ELISA. Sub-G1 cell percentages were analyzed by propidium iodide (PI) staining flow cytometry (FCM). ELISA assay Caspase-3 activity. Results: CAS increased the leakage of histone / DNA fragments in HeLa cells in a concentration-dependent manner (P <0.05), and significantly increased the percentage of Sub-G1 in HeLa cells (P <0.05). CAS activated Caspase-3 in HeLa cells in a Caspase-dependent manner (P <0.05). Conclusion: CAS can effectively induce HeLa cell apoptosis.