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目的建立过氧化氢(H2O2)诱导心肌细胞损伤模型,并在此模型上研究胡椒碱、胡椒酸甲酯、咖啡酸甲酯、尿嘧啶苷对心肌细胞损伤的保护作用。方法用H2O2诱导造成原代培养的心肌细胞损伤模型,将单体化合物分为3个剂量组,作用于正常和损伤的心肌细胞,利用全自动生化分析仪对细胞悬液中乳酸脱氢酶(LDH)和肌酸激酶同功酶(CK-MB)含量进行测定。结果回心草各单体成分对正常心肌细胞无明显影响。胡椒碱和胡椒酸甲酯各剂量组作用的心肌细胞悬液中LDH和CK-MB值与模型组对照有非常显著性差异(P<0.01),咖啡酸甲酯对CK-MB有非常显著性差异(P<0.01),尿嘧啶苷无显著性差异(P>0.05)。结论对H2O2诱导的心肌细胞损伤,胡椒酸甲酯和胡椒碱具有明显的保护作用,咖啡酸甲酯具有部分保护作用,尿嘧啶苷无保护作用。
Objective To establish a hydrogen peroxide (H2O2)-induced cardiomyocyte injury model, and to study the protective effects of piperine, methyl caprate, methyl caffeate, and uracil on cardiomyocyte injury in this model. Methods The primary cultured cardiomyocyte injury model induced by H2O2 was induced. The monomeric compounds were divided into three dose groups and applied to normal and injured cardiomyocytes. The Lactate dehydrogenase in the cell suspension was determined by an automatic biochemical analyzer. LDH) and creatine kinase isoenzyme (CK-MB) levels were determined. The results showed that the monomer composition of R. officinale had no significant effect on normal myocytes. The values of LDH and CK-MB in myocardial cell suspensions treated with piperine and methyl piperidinate were significantly different from those in the model group (P<0.01). Caffeic methyl ester was very significant in CK-MB. There was no significant difference (P<0.05) in uridine (P<0.01). Conclusion Methyl pepper and piperine have obvious protective effects on H2O2-induced cardiomyocyte injury. Methyl caffeate has partial protective effect and uridine has no protective effect.