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根据已发布的水稻黑条矮缩病毒(RBSDV)和南方水稻黑条矮缩病毒(SRBSDV)S7片段的序列分别设计特异性引物RB-S7-F/R和SRB-S7-F/R,采用RT-PCR、序列测定和同源性比对的方法,对2009年江苏发生的水稻矮缩病的病原进行了鉴定。结果表明,用RB-S7-F/R引物在47份检测样品中有36份可以扩增到一条1 200 bp左右的目的片段,而用SRB-S7-F/R引物未能扩增到预期条带。序列测定结果表明,扩增片段与已经发表的RBSDV中国分离物相应片段的核苷酸和氨基酸序列同源性分别为93.3%～100%和97.4%～100%,与SRBSDV相应片段的核苷酸和氨基酸同源性为77.4%～79.5%。系统进化分析得到类似结果。上述研究表明,2009年引起江苏水稻矮缩病症的病原为RBSDV,尚未发现SRBSDV的危害。
Specific primers RB-S7-F / R and SRB-S7-F / R were designed based on published sequence of RBSDV and SRBSDV S7, respectively. RT-PCR, sequence analysis and homology comparison were used to identify the pathogen of rice dwarf disease in Jiangsu in 2009. The results showed that 36 fragments of 47 samples could be amplified to a fragment of about 1 200 bp with RB-S7-F / R primer, while the expected fragment was amplified with SRB-S7-F / R primer Bands. Sequence analysis showed that the nucleotide and amino acid sequences of the amplified fragment were 93.3% -100% and 97.4% -100%, respectively, which were identical to those of the corresponding RBSDV isolates in China. And amino acid homology was 77.4% ~ 79.5%. Phylogenetic analysis yielded similar results. The above study shows that the pathogen causing dwarfing diseases of rice in Jiangsu in 2009 is RBSDV, and the harm of SRBSDV has not been found yet.