二、突变体的分离: 当分生孢子、孢子或菌丝碎片经过诱变,发芽生长分离出野生型和突变型之后,将发现突变体仅占很小的百分比(3％～4％)。因此,全材料筛选突变体需付出繁重的劳动。如果利用一定的分离技术可以减少分离的时间。但由于并非分离技术可用于每一个种,全材料分离法在一些情况下还要应用。在这里,介绍几种突变体的分离技术。 1、生长抑制法: 在丝状真菌中。涂布在琼脂平板培养基上的孢子或菌丝断片不可能以分离的单菌落形式生长,而是随菌丝萌发生长会很快的会合在一起。因此,就不容易将原来的接种物单独的分离出来。解决这个 Second, the separation of mutants: When the conidia, spores or hyphae fragments after mutagenesis, germination and growth of isolated wild type and mutant type, the mutant will find only a small percentage (3% to 4%). Therefore, whole-material screening mutants require heavy labor. If you use a certain separation technology can reduce the separation time. However, because of the separation techniques not available for each species, full material separation methods are also used in some cases. Here, we introduce the separation techniques of several mutants. 1, growth inhibition method: in filamentous fungi. Spores or mycelium fragments coated on agar plate medium can not grow as isolated single colonies, but will snap together quickly as the mycelial germination grows. Therefore, it is not easy to separate the original inoculum separately. Solve this