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目的:探索胚胎大鼠颞叶海马区神经元的改良培养方法。方法:在无血清培养基础上,采用改良的分离纯化法获取单细胞悬液,接种后在不同阶段通过加入不同配方的培养基进行培养,并进行免疫组化鉴定。结果:用该方法培养的颞叶海马区神经元细胞存活率高,生长状态良好,且βⅢ-tublin免疫染色为阳性,神经元细胞纯度可达90%以上。结论:改良分离、结合分阶段的不同培养条件是一种简单、高效的颞叶海马区神经元的纯化培养方法。
Objective: To explore an improved culture method of neurons in the hippocampus of the temporal lobe of embryonic rats. Methods: On the basis of serum-free culture, single cell suspension was obtained by the improved separation and purification method. After inoculation, the cells were cultured at different stages by adding different formulations of medium and identified by immunohistochemistry. Results: The neurons in hippocampus of temporal lobe cultured by this method showed high survival rate, good growth status and positive immunostaining for βⅢ-tublin. The purity of neurons was up to 90%. Conclusion: The method of purification and separation of neurons in the temporal lobe hippocampus is a simple and effective way to improve the separation and to combine different stages of culture.