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通过紫外-可见吸收光谱和荧光光谱滴定、稳态荧光猝灭以及盐效应实验研究了噻吩基钌配合物[Ru(bpy)2(Htip)]Cl2(1)、[Ru(Htip)2(dppz)]Cl2(2)、[Ru(Htip)3]Cl2(3)和[Ru2(bpy)4(H2bipt)]Cl4(4){bpy=2,2’-联吡啶;Htip=2-噻吩咪唑[4,5-f][1,10]邻菲咯啉;H2bipt=2,5-二(2-咪唑[4,5-f][1,10]邻菲咯啉)噻吩;dppz=二吡啶并[3,2-a:2’,3’-c]吩嗪}与酵母RNA(yeast-RNA)的相互作用,并比较了该类配合物与yeast-RNA和小牛胸腺DNA(ct-DNA)的键合性质。结果表明,该类噻吩基钌配合物是较强的RNA嵌入试剂,其中配合物2和3的RNA键合强度大于其DNA键合强度;此系列配合物在低盐和高盐浓度时均能与RNA较强地结合,即使在100 mmol/L Na Cl条件下仍具有较大的RNA键合常数;配合物1与RNA键合时荧光强度下降,配合物2在水溶液中以及与RNA键合时几乎无荧光,而它们与DNA作用时荧光强度明显增大,显示出良好的区分RNA和DNA的荧光特性。
The effects of Ru (bpy) 2 (Htip)] Cl2 (1) [Ru (Htip) 2] (dppz) on the fluorescence spectra of the thiophene ruthenium complexes were investigated by UV-vis absorption spectroscopy and fluorescence spectrometry titration, steady state fluorescence quenching and salt effect. )] Cl2 (2) [Ru (Htip) 3] Cl2 (3) and [Ru2 (bpy) 4 (H2bipt)] Cl4 (4) {bpy = 2,2’-bipyridyl; Htip = [4,5-f] [1,10] phenanthroline; H2bipt = 2,5-bis (2-imidazo [4,5-f] Pyridine [3,2-a: 2 ’, 3’-c] phenazine} with yeast-RNA and compared with yeast-RNA and calf thymus DNA -DNA). The results showed that the thienyl ruthenium complex was a strong RNA intercalating reagent, in which the RNA binding strength of complexes 2 and 3 was greater than that of DNA binding strength. Strong binding with RNA, even with 100 mmol / L Na Cl conditions still have a large RNA bonding constant; complex 1 and RNA binding decreased fluorescence intensity, complex 2 in aqueous solution and RNA binding Almost no fluorescence, and their fluorescence intensity significantly increased when interacting with DNA, showing a good distinction between the fluorescence properties of RNA and DNA.