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目的 探讨Penner 19空肠弯曲菌毒素 (CJT)对细胞毒性的影响。方法 (1)应用CHO及Vero细胞检测CJT毒性效应 ,以及CJT抗血清和GM1中和 /抑制作用 ;(2 )ELISA检测CJT结合GM1、CHO细胞受体 ,评价CJT抗血清及GM1中和或抑制作用。结果 (1)CJT对CHO和Vero细胞均具毒性 ,效应最低CJT量均为 3 12 5mg/L ,细胞变形率分别为 (5 7 3± 5 6 ) %及 (5 8 6± 5 2 ) % ,变形细胞中死亡率分别为 (5 3 8± 6 2 ) %及 (37 2± 6 9) %。对照组细胞变形率分别为 (8 6± 2 1) %及 (7 3±2 4 ) % ,显著低于CJT组 (P <0 0 1)。 (2 )与GM1呈阳性反应的CJT最低量为 1 5 6 3mg/L ,吸光度差值(P -N)为 0 2 4± 0 0 1(P >0 2 0 )。 (3)CJT抗血清可中和CJT毒性效应 ,其 1∶8及 1∶1倍稀释时 ,细胞变形率分别 <5 0 %和 <10 % ,与对照组比较差异有显著意义 (P <0 0 1)。 (4)GM1由 0 0 1增至 0 0 8μg/10 0 μl时 ,CJT致细胞形变率逐渐下降 ,继续增加GM1剂量 (0 0 8～ 0 6 4 μg/10 0 μl) ,细胞变形率却维持不变 ,且始终大于 5 0 %。 (5 )CJT结合CHO于作用后 3min最明显。CJT抗血清及GM1均可导致CJT结合CHO细胞能力减低。CJT抗血清完全抑制 ;而GM1部分抑制。结论 CJT对细胞可产生形态及致死?
Objective To investigate the effect of Penjn 19 Campylobacter jejuni toxin (CJT) on cytotoxicity. Methods (1) CHO and Vero cells were used to detect CJT toxicity and CJT antiserum and GM1 neutralization / inhibition; (2) ELISA detection of CJT binding to GM1 and CHO cell receptors, evaluation of CJT antiserum and GM1 neutralization or inhibition effect. Results CJT was toxic to both CHO and Vero cells with the lowest CJT levels of 3 12 5 mg / L and the cell deformability rates of (573 ± 56)% and (586 ± 52)%, respectively , Respectively. The mortality rates in deformed cells were (5 3 8 ± 6 2)% and (37 2 ± 6 9)%, respectively. The cell deformation rates in the control group were (86 ± 2 1)% and (73 ± 2 4)%, respectively, which were significantly lower than those in the CJT group (P <0.01). (2) The minimum amount of CJT which was positive for GM1 was 165 mg / L and the difference of absorbance (P -N) was 0 2 4 ± 0 0 1 (P> 0 20). (3) CJT antiserum could neutralize the toxic effects of CJT. The cell degeneration rates were <50% and <10% at 1: 8 and 1: 1 dilution, respectively, which was significantly different from the control group 0 1). (4) When the GM1 was increased from 0 0 1 to 0 0 8μg / 10 0 μl, the CJT induced cell degeneration gradually decreased and the GM1 dose continued to increase (0 0 8 ~ 0 64 μg / 100 μl) Remain unchanged, and always greater than 50%. (5) CJT binding to CHO at 3min after the most obvious. Both CJT antiserum and GM1 lead to a decrease in the ability of CJT to bind CHO cells. CJT antiserum completely inhibited; while GM1 partially inhibited. Conclusion CJT cells can produce morphological and lethal?