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以MT为基本培养基并附加各种植物激素和生理活性物质的一系列培养基中，6～8周龄的柑桔胚珠诱导产生愈伤组织和胚状体后进一步诱导形成次生胚状体，通过萌发或诱导丛芽再生出完整植株。外源激素的种类和组合对愈伤组织的诱导，胚状体的形成和次生胚状体的发生以及成苗都有显著的影响。染色体计数分析表明，通过次生胚胎发生途径的再生植株，其遗传性是稳定的。
In a series of medium with MT as basal medium supplemented with various plant hormones and physiologically active substances, citrus ovules from 6 to 8 weeks of age were induced to produce callus and embryoid bodies and then induced to form secondary embryoids The whole plant is regenerated by germination or inducement of shoots. The types and combinations of exogenous hormones have a significant effect on the induction of callus, the formation of embryoid bodies, the occurrence of secondary embryoids and seedling formation. Chromosomal counting analysis showed that the regenerated plants via the secondary embryogenesis pathways were genetically stable.