论文部分内容阅读
本项目用扩增片段长度多型性（ＡＦＬＰ）技术，研究了水稻光合作用和光保护机制有关的８个性状Ｆ_２代极端样本，在３２对引物的研究中，全部引物可以在亲本中检出多型性，有２８对引物可以在Ｆ_２群体，８组极端样本之间找到差异条带标记，每个性状可以找到７～２２个差异条带。在叶绿素ａｂ含量、玉米黄素＋花黄素总量、超氧化物歧化酶活性上，Ｌｅｍｏｎｔ具有明显的增效片段优势。玉米黄素＋花黄素＋紫黄质数总量和活性蛋白总量可能是提高光合作用的最大限制因子。同时研究表明，对预选ＰＣＲ产物进行纯化处理，可大大提高ＡＦＬＰ分析技术的有效性。
In this study, F 2 generations of 8 traits related to photosynthesis and photoprotection mechanism of rice were studied using amplified fragment length polymorphism (AFLP). In 32 pairs of primers, all the primers could be detected in the parents There were 28 pairs of primers that could be used to find different banding markers between F 2 population and 8 extreme samples, and 7-22 differential bands could be found for each trait. In the chlorophyll ab content, zeaxanthin + total amount of xanthophyll, superoxide dismutase activity, Lemont has obvious synergistic fragment advantage. The total amount of zeaxanthin + xanthophyll + violaxanthin and the total amount of active protein may be the maximum limiting factor for increasing photosynthesis. At the same time, research shows that the purification of preselected PCR products can greatly improve the effectiveness of AFLP analysis.