在优化的固定化条件下，通过戊二醛交联直接将青霉素酰化酶固定化。在优化的环境条件下测定游离酶和两种固定化酶的动力学常数。结果表明，尽管固定化酶的米氏常数增大，但产物抑制作用减弱，裂解青霉素的实验结果表明，固定化酶更适合在工业上应用。 Penicillin acylase is immobilized directly by glutaraldehyde crosslinking under optimized immobilization conditions. Kinetic constants of free enzyme and two immobilized enzymes were determined under optimized environmental conditions. The results showed that although the Mie constant of the immobilized enzyme increased, the product inhibition was weakened, and the experimental results of cleaving penicillin showed that the immobilized enzyme was more suitable for industrial application.