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目的探讨环孢素A在大鼠急性出血坏死性胰腺炎(ANP)时对脑组织损害的保护作用,从而为胰腺炎脑损伤的治疗提供实验依据。方法将60只SD大鼠随机分为正常对照组,诱导组和环孢素A处理组,经胰胆管逆行注射5%牛磺胆酸钠建立大鼠ANP模型,12h后处死动物,测定血清的TNFα水平,并检测脑组织含水量、脑组织MDA含量及脑微血管内白细胞聚集及附壁现象。结果经环孢素A处理后,血清中TNFα水平、脑组织含水量、脑组织MDA含量、脑微血管内白细胞聚集及附壁现象显著降低(P<0.01)。结论环孢素A可以减轻脑损害的发生和发展。
Objective To investigate the protective effect of cyclosporin A on brain injury induced by acute hemorrhagic necrotizing pancreatitis (ANP) in rats, and to provide an experimental basis for the treatment of brain injury induced by pancreatitis. Methods Sixty Sprague-Dawley rats were randomly divided into normal control group, induction group and cyclosporine A treatment group. Rat model of ANP was established by retrograde injection of 5% sodium taurocholate into the pancreaticobiliary duct. After 12 hours, the animals were sacrificed and serum TNFα levels, brain water content, brain MDA content and brain microvascular endothelial cell adhesion and adhesion phenomenon. Results After treatment with cyclosporin A, TNFα level, water content in brain tissue, MDA content in brain tissue, leukocyte accumulation in cerebral microvessels and the phenomenon of attachment were significantly decreased (P <0.01). Conclusion Cyclosporine A can reduce the occurrence and development of brain damage.