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NAC转录因子是近十年来新发现的具有多种生物功能的植物特异转录因子,在植物生长发育、激素调节和抵抗逆境等方面发挥着重要的作用。本研究基于Solexa技术对平榛花芽转录组文库进行分析,结合RACE-PCR扩增,从平榛中克隆了一个与NAC类基因同源的cDNA序列ChNAC1,该序列长度为1154bp,具有长度为876bp的完整开放阅读框架,推测编码蛋白含有291个氨基酸,具有N-末端同源性较高且十分保守的NAC结构域和一个位于C-末端的高度可变区域。qRT-PCR分析表明,ChNAC1可以在4℃低温胁迫条件下上调表达,在4h时出现表达峰值。组织表达分析结果表明,ChNAC1在雄花序中表达最高,其次是花芽、树皮和种子。推测ChNAC1可能参与植物响应低温反应过程。ChNAC1基因的克隆及表达分析为进一步阐明和探讨平榛NAC转录因子的功能奠定了基础。
NAC transcription factor is a newly discovered plant-specific transcription factor with many biological functions found in recent ten years, playing an important role in plant growth and development, hormone regulation and resistance to stress. In this study, based on the Solexa technique, we analyzed the Hamsil bud genomic transcriptome library. A cDNA sequence ChNAC1 homologous to the NAC gene was cloned by PCR from RACE-PCR. The cDNA sequence was 1154bp in length and had a length of 876bp Of the complete open reading frame, suggesting that the encoded protein contains 291 amino acids, with a N-terminal highly homologous and highly conserved NAC domain and a C-terminal highly variable region. qRT-PCR analysis showed that ChNAC1 was up-regulated at 4 ℃ under cold stress and peaked at 4h. Tissue expression analysis showed that ChNAC1 was the highest in male inflorescences, followed by flower buds, bark and seeds. Speculated that ChNAC1 may be involved in plant response to low temperature reaction. ChNAC1 gene cloning and expression analysis for further clarify and explore the function of hazel NAC transcription factor laid the foundation.