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目前间接免疫荧光试验(IIF)检测疟疾抗体用的抗原系由感染猴疟原虫或人疟原虫的夜猴制得。作者以培养的恶性疟原虫作抗原,对其敏感性、特异性和重复性等进行了观察,并与来自感染夜猴的恶性疟原虫抗原作了比较。当培养的恶性疟原虫血症达8%,其中环状体20%,滋养体35%,裂殖体45%时进行收集,以pH7.2的PBS洗5次,最后用同样的PBS制成悬液,涂制的抗原片厚血膜的每一高倍视野可见20～30个原虫,即以蜡纸封存于-70℃。感染夜猴的恶性疟原虫
Current Indirect Immunofluorescence Assays (IIF) Antigens for the detection of malaria antibodies are made from nocturnal monkeys infected with P. falciparum or P. falciparum. Using cultured P. falciparum as an antigen, the authors examined its sensitivity, specificity, reproducibility, and the like, and compared it to Plasmodium falciparum antigens from infected nocturnal monkeys. When P. falciparum was cultivated at 8%, 20% of the rings, 35% of trophozoites and 45% of schizonts were collected, washed 5 times with PBS at pH 7.2 and finally with the same PBS Suspension, coated antigen film Thick film per high power field can be seen 20 to 30 protozoa, which is sealed in wax paper at -70 ℃. Plasmodium falciparum infecting a night monkey