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以悬铃木的下胚轴和离体叶片为材料,研究了基因型、植物生长调节剂、光照条件、外植体部位等因素对其不定芽分化的影响。结果表明:1)6-BA与IBA的浓度比对下胚轴不定芽的分化影响较大,以6-BA∶IBA小于20∶1较为合适;而叶片的不定芽再生受基因型的影响显著,在4种供试材料中,PH2的再生能力最强,但0.1～1.0mg·L-1TDZ不利于其不定芽再生。2)黑暗培养对下胚轴的不定芽分化有一定的促进作用,但不利于叶片的不定芽再生,50～100lx的弱光有利于叶片的不定芽分化。3)不同部位的下胚轴切段及叶片的再生能力差异显著,其中靠近子叶的下胚轴切段和试管苗顶端的2枚叶片再生能力较强。此外,刻伤叶片的再生效果明显优于叶片切块,说明悬铃木的叶片与下胚轴的离体培养存在明显的生物全息现象。
Hypocotyls and leaves of Platanus acerifolia were used as materials to study the effects of genotypes, plant growth regulators, light conditions and explants on the differentiation of adventitious buds. The results showed that: 1) The concentration ratio of 6-BA to IBA had a greater effect on the differentiation of adventitious buds of hypocotyls, which was more suitable for 6-BA: IBA less than 20:1; however, the adventitious shoot regeneration of leaves was significantly affected by genotype Among the four tested materials, PH2 had the strongest regeneration ability, but 0.1-1.0 mg · L-1TDZ was not conducive to the regeneration of adventitious buds. 2) Dark culture can promote the adventitious bud differentiation of hypocotyls, but not conducive to the regeneration of adventitious buds. The weak light of 50 ~ 100 lx is conducive to adventitious bud differentiation of leaves. 3) The hypocotyl cuttings and the regeneration ability of the leaves differed significantly among different parts. Among them, two leaves near the cotyledon hypocotyl cuttings and the top of the test-tube shoots had stronger regeneration ability. In addition, the regenerating effect of engraved leaves was significantly better than that of the leaves, which indicated that there were obvious bio-holographic phenomena in vitro culture of Platanus acerifolia leaves and hypocotyls.