论文部分内容阅读
将金边阔叶麦冬 (LiriopeplatyphyllaWangetTangvar.variegataHort.)不同部位外植体块 ,接种于附加不同激素配比的MS培养基上 ,实验结果表明 ,取自根状茎芽点 ,培养基为MS +BA 1.5mg/L +NAA 0 .5mg/L的不定芽诱导率最高 ( 10 0 % ) ,长势较旺 ,不定芽和愈伤组织均较多 ,但极易造成性状分离。用MS +BA 3.0mg/L +NAA 0 .5mg/L液体培养可以直接“芽生芽” ,避免了脱分化过程 ,从而稳定保持嵌合性。不定芽每月可转接 1次 ,适宜的生根培养基为 1/2MS大量 +IBA 0 .2 5mg/L。试管苗在蛭石中炼苗后即可移栽。
The explants from different parts of Liriope platyphyllaWangetTangvar.variegataHort were inoculated on MS medium supplemented with different hormones. The experimental results showed that the explants were obtained from the shoots of rhizomes and the medium was MS + BA The induction rate of adventitious buds of 1.5mg / L + NAA0.5mg / L was the highest (10 0%), growing more vigorously, adventitious buds and callus were more, but it could easily cause trait separation. With MS + BA 3.0mg / L + NAA 0 .5mg / L liquid culture can be directly “shoot bud” to avoid dedifferentiation process, so as to maintain stable chimerism. Adventitious buds can be transferred once a month, suitable rooting medium is 1 / 2MS large amount + IBA 0. 25mg / L. Test tube seedlings can be transplanted in the vermiculite refining.