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目的 探索动物前庭适应产生的神经生理基础。 方法 采用胃电图波幅值作为观察植物神经反应的指标 ,利用小剂量、多次重复的 Coriolis加速度刺激 ,建立前庭适应动物模型。采用免疫组化染色和显微相对灰度分析方法 ,观察动物在产生前庭适应前后 ,以及前庭适应消退后 ,脑干前庭传出系统神经元细胞活性的变化 ,及其与胃电图波幅值变化的联系。 结果 建模成功豚鼠受到运动刺激时 ,刺激前后的胃电图波幅相近。在前庭适应的豚鼠身上 ,其脑干前庭传出系统神经元内胆碱乙酰化酶的含量明显增加 ;但在前庭适应消退后 ,这种含量的差别消失。这表明在豚鼠出现适应性时此神经元对于前庭终器感觉细胞的抑制性作用明显增强。 结论 脑干前庭传出系统神经元可能通过降低前庭终器感觉细胞向中枢神经系统的信号发放的程度 ,参与了适应性的产生过程。
Objective To explore the neurophysiological basis of vestibular adaptation in animals. Methods The amplitude of the electrogastrogram was used as an indicator to observe the autonomic nerve responses. The animal model of vestibular adaptation was established by small-dose repeated Coriolis stimulation. The changes of neuronal cell activity in the vestibular system of the brainstem vestibular system were observed by immunohistochemical staining and microscopic relative gray-scale analysis before and after the vestibular adaptation, Changing ties. Results When modeling successful guinea pigs, the amplitude of electrogastrogram was similar before and after stimulation. In the vestibular adaptation of guinea pigs, their brainstem vestibular system of neurons out of the content of choline acetyltransferase increased significantly; but in the receding vestibular adaptation, this content difference disappears. This indicates that the inhibitory effect of this neuron on vestibular end-effector cells is significantly enhanced in the presence of guinea pigs. Conclusion The neurons in the brainstem vestibular system may participate in the process of adaptive development by decreasing the signal distribution of sensory cells in the vestibular system to the central nervous system.