ATPase 是生物体内催化释放潜存于 ATP 分子中高能磷酸键内的能量.使之用于细胞内各种耗能功能的一类重要酶。对这类酶在体内的功能,调节及测定方法一直在细胞生理生化研究中占据重要位置。历来用于测定 ATPase 释放无机磷的主要方法是 Fiske Subborow 方法,但这种方法存在 ATP 及其它物质对生色过程的干扰,磷钢酸复合体不够稳定,以及灵敏度不够 ATPase is a kind of important enzyme that catalyzes the release of high-energy phosphate bond latent in the ATP molecule in vivo, which is used for various energy-consuming functions in the cell. The function, regulation and determination of these enzymes in vivo have always been important in the research of cell physiology and biochemistry. The main method that has traditionally been used to determine the release of inorganic phosphorus by ATPase is the Fiske Subborow method, but this method has the potential to interfere with the chromogenic process by ATP and other substances, the phosphates acid complex is not stable enough, and the sensitivity is not sufficient