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目的 确定土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因。方法 通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因 ;对差异表达基因进行DNA序列测定后于GeneBank内分析其同源性 ;应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。结果 通过抑制消减杂交共得到了 14条肿瘤组织差异表达的基因片段。其中 ,肿瘤组织和非瘤组织共有的 8条基因与GeneBank中的已知基因同源 ,肿瘤组织来源的 5条基因和非瘤组织来源的 1条基因在GeneBank中未找到同源基因。原位杂交差异基因在肝癌组织和非癌组织中均为特异性表达。结论 通过抑制消减杂交获得了 14条肝癌组织和非癌组织差异表达的基因 ,为理解HBV诱发肝癌的机理提供了一定的理论依据
Objective To determine the differentially expressed genes in hepatocarcinoma-induced hepatocellular carcinoma (HCC) and non-cancer tissues. Methods Differentially expressed genes in hepatocarcinoma and non-cancerous tissues induced by woodchuck HBV were cloned by suppression subtractive hybridization; DNA sequences of differentially expressed genes were analyzed and their homology was analyzed in GeneBank; differential genes were determined by in situ hybridization. Specific expression in hepatocellular carcinoma and non-cancerous tissues. Results A total of 14 gene fragments that were differentially expressed in tumor tissues were obtained by suppression subtractive hybridization. Among them, the 8 genes shared by the tumor tissue and the non-tumor tissue were homologous to the known genes in GeneBank. Five genes from tumor tissues and one gene from non-tumor tissues did not find homologous genes in GeneBank. In situ hybridization differential genes were specifically expressed in both HCC and non-cancerous tissues. Conclusion The differentially expressed genes in 14 HCC tissues and non-cancer tissues were obtained through suppression subtractive hybridization, which provided a theoretical basis for understanding the mechanism of HBV-induced HCC.