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目的 研究肝外胆管癌组织中N 乙酰氨基葡萄糖转移酶Ⅲ、Ⅳ、Ⅴ的活力及其意义。方法 通过反相高效液相层析 (HPLC)系统 ,测定 15例肝外胆管癌以及 15例良性胆道疾病组织中N 乙酰氨基葡萄糖转移酶Ⅲ、Ⅳ、Ⅴ (GnT Ⅲ、Ⅳ、Ⅴ )的活力。结果 肝外胆管癌组织中GnT Ⅲ、Ⅴ活力升高 ,其中GnT Ⅲ活力较良性组织增高约 3 3倍 ;GnT Ⅴ活力增高约 13 5倍 ,尤以伴淋巴结转移的肝外胆管癌为甚 (P <0 0 5 )。GnT Ⅳ活力变化不大。结论 N 乙酰氨基葡萄糖转移酶Ⅲ、Ⅴ活力升高可作为肝外胆管癌存在或转移的标志物 ,有助于肝外胆管癌的诊断及预后判断。
Objective To study the activity and significance of N-acetylglucosamine transferase III, IV, and V in extrahepatic cholangiocarcinoma. Methods The activity of N-acetylglucosamine transferase III, IV, V (GnT III, IV, V) in 15 cases of extrahepatic cholangiocarcinoma and 15 cases of benign biliary disease was measured by reversed-phase high performance liquid chromatography (HPLC) system. . Results The activity of GnT III and V increased in extrahepatic cholangiocarcinoma tissues, and the GnT III activity was about 33 times higher than that of benign tissue. The GnT V activity was about 13 5 times higher, especially in extrahepatic bile duct carcinoma with lymph node metastasis ( P <0 0 5 ). GnT IV activity changes little. Conclusion The increased activity of III and V of N-acetylglucosaminyltransferase can be used as a marker for the presence or metastasis of extrahepatic cholangiocarcinoma, which contributes to the diagnosis and prognosis of extrahepatic cholangiocarcinoma.