小青龙汤和人参五味子汤二步序贯治疗对哮喘小鼠呼吸道炎症与重塑的影响

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目的探讨小青龙汤和人参五味子汤二步序治疗贯治疗对哮喘小鼠呼吸道炎症与重塑的影响。方法健康雌性昆明系小鼠40只,随机分为4组(每组10只):正常对照组、哮喘组、布地奈德组和二步序贯治疗组。采用卵清蛋白建立小鼠哮喘模型;在致敏阶段,二步序贯治疗组小鼠胃饲人参五味子汤;在激发阶段,布地奈德组小鼠在激发前雾化吸入布地奈德,二步序贯治疗组予以小青龙汤胃饲。采用普通光镜观察哮喘小鼠呼吸道组织病理变化确定呼吸道炎症;免疫组织化学法测定基质金属蛋白酶-9(MMP-9)、基质金属蛋白组织酶抑制剂-1(TIMP-1)水平,确定呼吸道重塑程度。结果正常对照组小鼠呼吸道组织颜色呈粉红色,其余各组小鼠呼吸道组织较正常对照组略苍白,以哮喘组较明显,部分出现黑色斑点或纹理,体积增大。布地奈德组和二步序贯治疗组呼吸道组织炎症改变均较哮喘组明显减轻,差异有统计学意义(Pa<0.01);但二组较正常对照组明显加重,差异有统计学意义(Pa<0.01);布地奈德组与二步序贯治疗组呼吸道炎症改变比较差异无统计学意义(P>0.05)。布地奈德组和二步序贯治疗组呼吸道MMP-9、TIMP-1表达水平和MMP-9/TIMP-1明显低于哮喘组,差异有统计学意义(Pa<0.01);但二组与正常对照组比较,呼吸道MMP-9和MMP-9/TIMP-1明显增高,差异均有统计学意义(Pa<0.01);布地奈德组与二步序贯治疗组各指标差异均无统计学意义(Pa>0.05)。结论二步序贯治疗对哮喘小鼠具有抑制呼吸道炎症和重塑的作用,在一定程度上可以替代布地奈德吸入治疗。 Objective To investigate the effects of Xiaolonglong Decoction and Ginseng Decoction on the airway inflammation and remodeling in asthmatic mice. Methods Forty female Kunming mice were randomly divided into 4 groups (n = 10): normal control group, asthma group, budesonide group and two-step sequential treatment group. Ovalbumin was used to establish a mouse model of asthma. During the sensitization phase, mice in the two-step sequential treatment group were given gastric gavage of ginseng and Wuwei Zi Tang. At the excitation stage, the budesonide group mice were challenged with inhaled budesonide Step-sequential treatment group to small dragon soup stomach feeding. The pathological changes of airway in asthmatic mice were observed with routine light microscope, the airway inflammation was determined, the levels of MMP-9 and TIMP-1 were detected by immunohistochemistry, Reshaping. Results In normal control group, the color of airway of mice was pink, while the rest of the mice in each group were slightly pale. The airway of the mice in the other groups was more pale than the normal control group. The black spots or texture were observed in the asthma group and the volume increased. Budesonide group and two-step sequential treatment group were significantly lower than the asthma group, the difference was statistically significant (P <0.01), but the two groups were significantly higher than the normal control group, the difference was statistically significant (Pa <0.01). There was no significant difference in respiratory inflammation between budesonide group and two-step sequential treatment group (P> 0.05). The levels of MMP-9, TIMP-1 and MMP-9 / TIMP-1 in budesonide group and two-step sequential treatment group were significantly lower than those in asthma group (P <0.01) The levels of MMP-9 and MMP-9 / TIMP-1 in the respiratory tract were significantly higher in the normal control group (all P <0.01), but there was no significant difference between the budesonide group and the two-step sequential therapy group Significance (Pa> 0.05). Conclusions Two-step sequential therapy can inhibit airway inflammation and remodeling in asthmatic mice, which can replace inhalation of budesonide to a certain extent.
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