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以离子交换和反相固定相构成的混合固定相电色谱中 ,溶质迁移在受到疏水、离子交换作用的同时 ,对于带电溶质而言 ,还受到电泳迁移的影响。根据离子独立迁移原理 ,结合色谱过程中的多种相互作用 ,得到了描述溶质表观迁移速率与其各形态迁移速率、各种相互作用之间相互关系的理论表达式 ;讨论了混合模式电色谱中流动相的 pH及其中的有机调节剂浓度、混合固定相配比等对电渗流的影响及不同形态溶质在柱内的输运特征。结果表明 ,在电色谱中采用混合固定相可以在较大的 pH和有机调节剂浓度范围内得到较强且稳定的电渗流。pH通过改变溶质的形态影响分离 ;有机调节剂对中性溶质的影响满足一般反相电色谱中的规律 ;竞争试剂对带电溶质分离的影响较大 ,它的加入可以有效地改善峰形 ,但是由于电泳作用的调节 ,其作用并不如一般离子交换色谱中对选择性的影响大
In mixed fixed-phase electrochromatography consisting of ion-exchange and reversed-phase stationary phases, solute transport is affected by electrophoretic migration for hydrophobic solutes as well as hydrophobic and ion-exchange interactions. According to the principle of independent ion migration and the combination of many kinds of interactions in the chromatographic process, a theoretical expression describing the relationship between the apparent solute transfer rate and the migration rates of various forms and the various interactions is obtained. In the mixed mode electrochromatography PH of the mobile phase and organic modifier concentration, mixed stationary phase ratio on the electroosmotic flow and transport characteristics of different morphological solutes in the column. The results show that the mixed stationary phase in electrochromatography can obtain a strong and stable electroosmotic flow at a large pH and organic modifier concentrations. The influence of pH on the neutral solute can be affected by the change of solute morphology. The effect of organic modifier on neutral solute can meet the general rule of reversed-phase electrochromatography. The competition reagent has a great effect on the separation of charged solute and its addition can effectively improve the peak shape. Due to the regulation of electrophoresis, its effect is not as good as selective ion exchange chromatography