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利用基于混合线性模型的复合区间作图法对重组近交系(recombinant inbreed line,RIL)“中G6”进行QTL定位,生育期性状共定位了主效QTL位点5个,上位性QTL位点4对,纤维品质性状定位了主效QTL位点1个,上位性QTL位点6对,产量性状定位了主效QTL位点3个,上位性QTL位点5对。其中定位的果枝始节、吐絮期、上半部平均长度、衣分的主效QTL位点均距离最近标记1cM以下,这有利于在育种实践中主效QTL跟踪检测。定位的霜前花率主效QTL位点具有较高的加性效应和遗传贡献率,应进行QTL精细定位、图位克隆,将会对早熟性育种工作有一定的推动意义。定位的马克隆值、衣分和子指总的遗传贡献率均在30%以上,对性状特征均影响显著。对主效及上位性QTL位点进行遗传效应分析,验证了前人有关数量性状遗传符合主基因与多基因混合遗传的论断,认为此模型是研究数量性状遗传的有效途经;对主效及上位性QTL位点进行A、D亚基因组定位,并对主效及上位性QTL位点在A、D亚基因组上的分布及互作方式进行了详细的分析。全文认为上位性QTL位点和主效QTL位点一样在物种遗传变异和聚合育种中起着重要作用。
The QTL mapping of recombinant inbreed line (RIL) “G6 ” was performed by composite interval mapping based on mixed linear models. Five main QTLs were mapped during the growth traits. The epistatic QTLs There were four pairs of QTLs for fiber quality traits, one major QTL for fiber quality traits, six pairs of epistatic QTLs, three major QTLs for yield traits, and five pairs of epistatic QTLs for yield traits. Among them, the QTL locus of fruiting branches, boll opening, the average length of the upper half, and the major loci of the lint are all below 1cM, which is favorable for tracing the major QTLs in breeding practice. The major QTLs for locating frost before frost have higher additive effects and genetic contribution rate. Fine QTL mapping and map cloning should be helpful for premature frostbite breeding. The value of micronaire localization, the total genetic contribution of lint and sub-index were more than 30%, which had a significant impact on traits. The main effect and epistatic QTL loci genetic effect analysis to verify the predecessor of quantitative traits consistent with the main gene and polygenic inheritance of the conclusion that this model is an effective way to study the quantitative trait genetic; Sex QTLs for A and D subgenome loci. The distribution and interaction of main and epistatic QTLs on A and D subgenomes were analyzed in detail. The full text of the view that the epistatic QTLs and major QTLs play an important role in species genetic variation and polymerization breeding.