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目的:研究人B7-2基因修饰的食管癌细胞作为瘤苗的抗肿瘤作用。方法:将真核荧光表达载体pEGFP-C3-B7-2,通过脂质体转染技术转染人食管癌细胞株EC9706。外周血来源的树突状细胞(DC)负载肿瘤抗原后,与自体T淋巴细胞共培养3d,获得细胞毒性T淋巴细胞(CTL)。用四甲基偶氮唑蓝(MTT)法检测CTL对转染和未转染pEGFP-C3-B7-2的人食管癌细胞EC9706的杀伤活性。结果:CTL对转染pEGFP-C3-B7-2的肿瘤细胞的杀伤活性大于转染pEGFP-C3和未转染细胞的杀伤活性(P<0.05)。结论:人B7-2基因修饰的EC9706肿瘤细胞瘤苗,可诱导出明显的抗EC9706细胞的免疫效应。
Objective: To study the antitumor effect of human B7-2 gene modified esophageal cancer cells as tumor vaccine. Methods: The eukaryotic expression vector pEGFP-C3-B7-2 was transfected into human esophageal carcinoma cell line EC9706 by lipofectamine 2000. Dendritic cells (DCs) derived from peripheral blood were loaded with tumor antigens and co-cultured with autologous T lymphocytes for 3 days to obtain cytotoxic T lymphocytes (CTLs). The killing activity of CTL on human esophageal cancer cell EC9706 transfected and non-transfected pEGFP-C3-B7-2 was detected by methyl thiazolyl tetrazolium (MTT) assay. Results: The killing activity of CTL on tumor cells transfected with pEGFP-C3-B7-2 was greater than that of transfected pEGFP-C3 and untransfected cells (P <0.05). Conclusion: Human B7-2 gene modified EC9706 tumor cell vaccine can induce significant immune effect against EC9706 cells.