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研究谷胱甘肽 S 转移酶(glutathioneS transferase,GST)M1和T1基因多态性与支气管哮喘(asthmabronchial)的关系。采取聚合酶链反应对60名支气管哮喘患者和60名正常对照进行了GSTM1和GSTT1基因非缺失(+)和缺失(0)等位基因分布频率研究。结果表明,与对照组相比,支气管哮喘患者GSTM1基因缺失的纯合子(0/0)频率(81 2%)显著升高(χ2=32 46,P<0 001;wχ2=28 75,P<0 001)。对于GSTT1也得到类似资料。而支气管哮喘患者GSTT1基因缺失等位基因(0/0)频率(71 7%)比对照组(11 7%)显著升高(χ2=26 72,P<0 001;wχ2=35 75,P<0 001)。表明GSTM1、GSTT1缺失等位基因纯合性在哮喘患者中是最有特征性的。GSTM10/0、GSTT10/0结合的频率患者组为61 7%,对照组仅为1 7%(χ2=27 3,P<0 001)。提示GSTM1和GSTT1基因多态性与哮喘有显著性关联,两个基因的突变可以被视为发生支气管哮喘遗传风险因子。
To investigate the relationship between glutathione S transferase (GST) M1 and T1 gene polymorphism and asthmabronchial. Polymerase chain reaction (PCR) was performed on 60 asthma patients and 60 normal controls for allelic frequencies of the non-deletion (+) and deletion (0) alleles of GSTM1 and GSTT1 genes. The results showed that the frequency of homozygous (0/0) deletion of GSTM1 gene in patients with bronchial asthma was significantly higher than that in controls (81 2%) (χ2 = 32 46, P <001; wχ2 = 28 75, P < 0 001). Similar information is obtained for GSTT1. The frequency of GSTT1 deletion allele (0/0) in bronchial asthma patients was significantly higher than that in controls (71.7%) (χ2 = 26 72, P <001; wχ2 = 35 75, P < 0 001). This indicates that the homozygosity of GSTM1 and GSTT1 deletion alleles is the most characteristic in asthmatic patients. The frequency of GSTM10 / 0, GSTT10 / 0 binding was 61.7% in the patient group and only 17% in the control group (χ2 = 273, P <0.001). It is suggested that there is a significant association between GSTM1 and GSTT1 gene polymorphisms and asthma. Mutations of the two genes may be considered as genetic risk factors for bronchial asthma.